NHS Activated Agarose, Pierce™ (2023)

Article 1 General

1.1 In these terms and conditions, the following terms have the following meanings:
(a) Terms: these terms and conditions;
(b) Proveedor: VWR International B.V., si
apply to their legal successors;
(c) Customer: any (legal person) who has made or entered into a contract with the Supplier, or to whom or on behalf of the Supplier an offer or quotation has been issued or issued, or to whom or on behalf of
Goods delivered or delivered by or on behalf of the Supplier, or one or more services performed by or on behalf of or for the benefit of the Supplier.

1.2 All offers, quotes issued, agreements concluded and their execution by or on behalf of the Supplier, as well as all deliveries and services carried out by or on behalf of the Supplier, will be
be bound by these terms.

1.3 Deviations from these terms and conditions apply only if the Provider and the Customer agree in writing.

1.4 The application of general terms and conditions other than these terms and conditions, including the conditions of purchase and/or other general terms and conditions or used by them, is expressly excluded.
customer. The customer, by accepting an offer or quote made by or on behalf of the supplier, accepts the applicability of these terms and conditions, to the exclusion of other general terms and conditions, to the
Enter into an agreement with a supplier to accept delivery of goods by or on behalf of a supplier, or to accept services provided by or on behalf of a supplier.

Article 2 Quotation

2.1 Any offer or quotation made by or on behalf of the Supplier is without obligation and is not binding on the Supplier, unless the Supplier expressly states otherwise in writing or the parties otherwise agree in writing.

2.2 Price lists, brochures, catalogues, brochures and other data (including in digital form) provided by or on behalf of the provider have been compiled with the greatest possible care, but are binding on the provider.
Only if the provider expressly confirms this in writing.

2.3 All data and images related to an offer or an offer remain the exclusive property of the provider. Without the prior written consent of the provider, the customer may not
Copy or use any material in whole or in part. The use of this material must be limited to the client's own use in case of placing an order with the supplier. first request
All Supplier materials must be returned to Supplier immediately.

2.4 If no agreement is reached, the supplier is entitled to charge the buyer a reasonable fee in relation to the offer or quotation.

Article 3 Establishment and content of the agreement

3.1 When the provider accepts the transfer or the customer's order, an agreement is concluded between the provider and the customer, the scope and content of which is effective from the date of acceptance.
by the supplier. Orders or assignments made by the Customer to the Supplier are considered binding and irrevocable upon receipt by the Supplier.
In writing, by electronic or telephone means or in any other way, unless the request or the corresponding assignment is conditioned to its expression.

3.2 The Provider is only bound by an agreement entered into by one or more persons who have the authority to bind the Provider.

3.3 Changes or cancellations of orders or transfers in whole or in part can only be made with the provider's prior written consent, provided that the provider has executed
Activities are reimbursed by the buyer. In case of change or cancellation of an order or transfer, the Client is obliged to pay the Provider the charges corresponding to the first request of the Provider, and
Supplier reserves the right to charge Buyer for these charges and reschedule delivery when applicable.

(Video) Packing of a Gravity Flow Column for Protein Purification

3.4 The adaptation or modification of the contract can only be carried out through documents signed by both parties.

3.5 For jobs or jobs for which, due to their nature and/or size, a quote or order confirmation has not been sent, it is agreed that the Supplier or Supplier
Start delivery.

3.6 The agreement between the parties is concluded on the condition that the Supplier and the other contractual partners of the Supplier fulfill their obligations in a timely and correct manner.

3.7 The Supplier is entitled, after signing the Agreement, at least until the Supplier begins to perform the Agreement or continues to perform
The client is obliged to provide adequate guarantees regarding the timely fulfillment of its obligations by the client.

3.8 The Provider is authorized to engage third parties for the performance of the Agreement. This fee will be passed on to the client.

Article 4 Prices and costs

4.1 Unless otherwise agreed by the parties in writing, each offer from the supplier is without obligation.

4.2 The prices indicated by the Supplier in any way are always exclusive of VAT and any additional charges, including, but not limited to, shipping costs, administration costs, etc., unless otherwise stated.
The Supplier is entitled to an administrative charge if the delivered goods do not exceed a specific invoice value determined by the Supplier.

4.3 At the specific request of the customer, the supplier may provide the customer with goods and/or services that are not within the scope of the supplier's policy (third party procurement). there
In such a case, all costs involved (including, but not limited to, personnel costs), regardless of their amount, will be invoiced to the Customer by the Provider.

4.4 Any fees charged by the Provider to the Provider will be passed on to the Customer by the Provider. This also applies in particular to the acquisition of goods by third parties.

4.5 For the delivery of ethanol, the supplier will charge the established fiscal surcharge. If the buyer provides the supplier with a
Obtaining a GST exemption permit allows deliveries without GST. All costs associated with related documentation, including but not limited to personnel costs, shall be borne by
Provider to customer.

4.6 Supplier shall reasonably charge Buyer additional charges for Goods requiring temperate transportation, including, without limitation, transportation at specified temperatures.

4.7 At the customer's request, the supplier may provide the customer with a declaration on the origin, composition and/or identity of the delivered goods. providers can
Charge a reasonable fee for issuing such notices to customers.

4.8 The Supplier may provide certain services at the Customer's request, including but not limited to equipment installation, advice, generation of applications and/or publication of certain documents, etc.
The Supplier may charge the Buyer a reasonable fee for the services provided by the Supplier.

Article 5 Risk

5.1 The risk of the goods that are the subject of an agreement between the supplier and the customer will pass to the customer at the time of delivery of these goods to the customer.

(Video) How to use Pierce Protein Concentrators for 100-500 µL sample volumes

5.2 The costs of unloading the merchandise and eventual assemblies, installations and commissioning are always borne by the customer.

Article 6 Delivery

6.1 Unless otherwise agreed in writing, delivery will be made promptly (freight unpaid) to the customer's business (or domicile) or other place designated by the customer.

6.2 Unless otherwise agreed in writing, the delivery period is the time when the goods in question reach the customer. This also applies if the supplier returns the product.
The delivery must be assembled, installed and/or commissioned.

6.3 Any shortages, defects and damages must be reported by the buyer to the supplier in writing within 24 hours of delivery, otherwise the goods are deemed to be delivered undamaged.
Complete and without damage or breakdowns has reached the customer.

6.4 The Supplier is entitled to partial deliveries, in which case the parts may be invoiced separately. In such a case, the customer is required to pay a separate invoice as specified here

6.5 The supplier will try to shorten the delivery period as much as possible. The supplier is obliged to comply as far as possible with the specified delivery times or deadlines, whereby the customer acknowledges that the supplier
The delivery time depends on your own provider. If the supplier exceeds the agreed delivery period, the customer must notify the supplier in advance of the breach of contract
The provider will default in this regard. The supplier will not be liable to the client for any damage caused by exceeding the time or delivery deadline. Also, more than
Delivery terms or delivery times The buyer has no right to cancel or withdraw from the contract or refuse to accept the goods, unless the buyer sets a reasonable deadline for the supplier in advance.

6.6 The supplier may charge the customer for the goods that the customer does not collect on time and store them at the customer's expense. In addition, he does not deliver the purchases on time.
The buyer ordering the goods has the right to withdraw from the contract without prejudice to the supplier's right to claim damages.

6.7 Delivery of the goods is made at the location most convenient for the Supplier, at the Supplier's choice. In special cases, the customer may specify a specific place of delivery. In that case it will be
The supplier instructs the carrier to deliver the goods to the agreed place to the extent possible, provided this does not create any legal or practical obstacle and does not result in loss of time or
higher costs If necessary, the supplier will quote separately for the special requirements of the client.

Article 7 Transport and Packaging

7.1 The Supplier is free to decide how to pack, transport and/or ship the Goods, subject to due observance of legal requirements.

7.2 The cost of any specific wishes of the customer regarding packaging and/or shipping and/or shipping shall be borne by the customer. Providers have the right to fulfill specific wishes at any time.
Buyer's regulations regarding packaging and/or shipping and/or shipping.

Article 8 Packaging

8.1 Only durable packaging in good and usable condition may be removed by the buyer's supplier at cost, and only if this packaging is disposed of separately in accordance with the following conditions
The amount of the cost price will be indicated on the delivery note or on the invoice.

8.2 If the buyer returns the packaging (including but not limited to return pallets and drums) to the supplier in a condition that is not as good as the packaging at the time of delivery
The supplier is entitled to charge a reasonable fee for the purchase of related products. The customer is not entitled to compensate the value of the package in whole or in part at any time
What you owe to your suppliers. In addition, the Provider reserves the right to charge a holding fee if certain packages are not returned on time.

Article 9 Force majeure

(Video) Immunoprecipitation

9.1 In the event of force majeure, the provider is not obliged to fulfill its obligations, provided that the provider informs the customer of the occurrence within a reasonable time. In this case, the provider can choose a
Specify new delivery terms and/or new conditions or terminate the contract.

9.2 Force majeure referred to in the preceding paragraph is understood to be any circumstance beyond the Supplier's control that prevents timely or complete compliance with the Supplier's obligations in time or in perpetuity.
so impede or hinder that the supplier cannot reasonably be expected to comply in a timely or complete manner.

Article 10 Warranty

10.1 The supplier takes due care of the ingredients of its usual range. However, the supplier does not offer any guarantee for the goods it supplies. The provider, upon request
Refer the complaints or claims of the clients under guarantee to the suppliers of the suppliers or producers of the goods in question.

10.2 The Provider will use its best ability to provide any service to the Customer, but does not make any warranty in this regard.

Article 11 Complaints

11.1 Any customer complaint will only be dealt with by the supplier if it is received in writing by the supplier within 10 (ten) days of delivery.

11.2 If the supplier accepts the claim, the supplier will guarantee compensation and/or replacement of the goods in question. Any compensation is limited to the invoice amount.
The supplier has shipped or will ship the delivered goods to the customer.

Article 12 Return requirements

12.1 Only if the supplier agrees in advance to the return and the return is accompanied by
Indicate the return order number provided by the supplier.

12.2 Return requests will only be accepted if they are made in writing within 10 (ten) days of delivery of the relevant goods. Only when the merchandise is in
Close the original packaging, if the original packaging is not damaged, written on, glued or otherwise damaged.

12.3 Goods that do not belong to the regular scope of the supplier and goods that are specially purchased and delivered by the supplier at the request of the customer cannot be returned. Also, there are no returns.
When the best before date has expired or expires within 14 (fourteen) days after delivery.

12.4 Supplier may charge Buyer a reasonable fee in connection with (acceptance of) returns and related stock of the Goods.

Article 13 Payment

13.1 Unless the parties agree otherwise in writing, the Client shall pay the invoice sent by the Supplier within 14 (fourteen) days following the date of the invoice in the manner indicated on the invoice.

13.2 If the invoice is not paid within the period specified in the preceding paragraph or otherwise agreed in writing by both parties, the customer is in breach of contract in accordance with the law.
An additional notice of default will be required. In such case, the Customer will owe the Provider 1% monthly interest from the due date of the corresponding invoice.

(Video) Covalent Coupling of Antibodies to Magnetic Beads - Fast and Efficient

Article 14 Reservation of title

14.1 Without prejudice to the provisions of these Terms and Conditions relating to the transfer of risk, the provider retains ownership of the delivered goods until the customer has fulfilled his obligations in full.
In accordance with the relevant contract, including full payment of the invoice sent by the supplier to the customer for the relevant delivery.

14.2 The customer agrees to fully cooperate with the supplier now if the supplier wishes to recover the delivered goods.

Article 15 Legal regulations/instructions

15.1 for
supplier. The provider assumes no liability in this regard and releases the customer from any liability in this regard.

15.2 The Supplier is not responsible for the Client's compliance with applicable laws and regulations, including those mentioned in the previous paragraph.

15.3 The customer must be duly informed about the permitted application of the ordered and delivered goods, and the customer must comply with the instructions of the manufacturer of the goods in question.

Article 16 Liability

16.1 Any liability of the supplier is limited to the amount of the invoice that the supplier has or will send to the customer for the goods delivered. In addition, the supplier's responsibility
limited to direct damage. The provider is never liable for consequential and consequential damages.

16.2 The provider is only liable for damage caused intentionally or through the provider's gross negligence. The provider's liability is in no way limited to the amount paid
The event in question is covered by the provider's insurance, so a sequence of events that occur in a particular situation will be called an event for the purposes of this definition.

16.3 The customer must report any damage to the supplier within fourteen (14) days of the damaging incident. Notices to providers under this section shall be
Made by certified mail. If the customer does not report the damage to the provider immediately or in the prescribed manner, the provider is not under any circumstances obliged to compensate the damage.

16.4 Without prejudice to the provisions of these Conditions, the provider will not be responsible for damages caused by incorrect or inappropriate use of the goods in question.

16.5 Any advice given by the Provider to the Customer is voluntary and non-binding, and the Provider shall not be liable for it. Said advice does not relieve the client of the obligation to determine the suitability of it.
Please test the delivered goods before using them, and use the related goods correctly. The provisions of this paragraph also apply to other services and services provided by the Provider, such as
Installation of equipment, etc.

16.6 The Buyer indemnifies the Supplier against third party claims for the use of the goods delivered to the Buyer by the Supplier.

Article 17 Applicable law and choice of forum

17.1 The legal relationship between Supplier and Customer, including quotations, orders, agreements and deliveries, is exclusively governed by Dutch law.

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17.2 All disputes between the Provider and the Customer will be referred to the competent court in Amsterdam.

These general terms and conditions were last sent to the
Amsterdam Chamber of Commerce, number DS04038098.


NHS Activated Agarose, Pierce™? ›

The Thermo Scientific Pierce NHS-Activated Agarose Resin allows simple and efficient covalent immobilization of proteins to a beaded-agarose support, providing a valuable tool for affinity purification of antibodies, antigens or other biomolecules.

What is pierce NHS-activated agarose dry? ›

NHS- activated agarose reacts with primary and secondary amines to form stable amide and imide linkages respectively. Most proteins can be coupled in 30 mins with greater than 85% coupling efficiency. No hazardous chemicals are used, and the agarose is adaptable to use with spin columns, batch methods, or FPLC.

What is NHS-activated agarose slurry? ›

NHS-Activated Agarose consists of 6% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligands that contain primary amines.

What is NHS-activated? ›

NHS-activated agarose is crosslinked, 6% beaded agarose resin that contains N-hydroxysuccinimide (NHS) functional groups. The activated resin reacts with primary amines to form stable amide linkages that covalently immobilize antibodies or other proteins for use in affinity purification procedures.

What is NHS-activated Sepharose? ›

NHS-activated Sepharose® 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

What are the 2 types of gel agarose? ›

Two major types of agarose are commercially available: hydroxyethylated agaroses, which form gels with lower gel strength and significantly lower melting and gelation temperature, and gels cast from unmodified agarose.

What is the difference between SDS gel and agarose gel? ›

Agarose gel electrophoresis results in low resolution. SDS PAGE gives a better resolution than agarose. Ethidium bromide is the commonly used stain. Silver or coomassie staining technique is used.

What is NTA agarose? ›

Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine.

What is NHS used for? ›

The NHS was set up in 1948 to provide everyone in the UK with healthcare based on their needs, and not on their ability to pay. The NHS is respected throughout the world for the standard of care it gives to patients.

Why is NHS a good leaving group? ›

The anion of NHS ester is abbreviated as O-succ. In the mechanism, the carboxylate ion acts as a nucleophile and attacks at the carbonyl carbon of the given reactant which leads to formation of an intermediate and anion of NHS ester is stable and acts as a good leaving group in next step.

Is NHS soluble in water? ›

NHS is membrane-permeable and water-soluble. NHS is soluble in organic solvents, as is Sulfo-NHS to a lesser extent. Because NHS is a leaving group in reactions with primary amines, the final conjugation product resulting from NHS and Sulfo-NHS reactions is identical.

How do you activate agarose? ›

Agarose can be activated by adding cyanogen bromide, dissolved in acetonitrile, to beads suspended in a solution of sodium carbonate.

What is Sepharose used for? ›

Abstract. Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.

What is NHS coupling? ›

6.3 Coupling reactions involving N-hydroxysuccinimide (NHS) ester derivative. As NHS is highly reactive at physiological pH, it is used for amine coupling reactions in bioconjugation. As shown in Fig. 8.5, the NHS ester compounds react with nucleophiles to form an acylated product with NHS as a leaving group.

What is the difference between 1% and 2% agarose gel? ›

Higher percentage gels are better for separating smaller DNA fragments. The photos below compare the same DNA fragments run for the same amount of time on a 2% gel vs. a 1% gel. Notice that separation of the smaller fragments (200-50 bp) is much better on the 2% gel.

What is difference between agar and agarose? ›

Agarose is also used in the field of microbiology, but to a much lesser extent than agar. While Agar is extracted directly from the cell wall of different species of red algae (Gelidium, Gracilaria...), Agarose is obtained later from the agar through a complex and costly purification process.

Why is 2% agarose gel used? ›

For a standard agarose gel electrophoresis, a 0.8% gel gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel gives good resolution for small 0.2–1kb fragments. 1% gels is often used for a standard electrophoresis.

Why agarose is not used in SDS? ›

One more answer for your question here. For protein in SDS gel, we normally run longer time right. If you use agarose gel, it will melt before your getting your results. Sometimes, at the end of SDS page running time, you may notice the running solution is hot.

Why is SDS not required in a DNA agarose gel? ›

Agarose gels for DNA electrophoresis

DNA is always negatively charged in solution. With proteins, you had to use SDS to ensure that all proteins had the same negative charge density, but you don't need to use SDS with your DNA. DNA doesn't usually have tertiary structure.

Why use polyacrylamide gel instead of agarose? ›

Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of DNA whose lengths differ by as little as 0.1% (i.e., 1 bp in 1000 bp). (2) They can accommodate much larger quantities of DNA than agarose gels.

What are NTA agarose beads charged with? ›

Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine.

What is the difference between Ni-NTA and Ni Ida? ›

Our comparative study shows that Ni-NTA is more robust than Ni-IDA at higher concentrations of EDTA. Ni-NTA had a non-linear overall drop in binding capacity of 46%, with slightly steeper decay after 1 mM EDTA. Up to 1 mM EDTA, the binding capacity of Ni-IDA decays similar to that of Ni-NTA.

What size are Ni-NTA agarose beads? ›

Ni-NTA Agarose beads are approximately 45-165 µm, Ni-NTA Superflow beads range from 60-160 µm and Ni-NTA Magnetic Agarose Beads are between 20-70 µm in diameter.

What is the advantage of NHS? ›

Without a doubt, the NHS is one of the fairest healthcare systems in the world as by sharing the costs via taxation, high-quality healthcare can be administered to every one according to their need. Since 1948 the NHS has shown that it can adapt to changes in the medical field.

What is NHS and why is it good? ›

The National Honor Society recognizes high schoolers who meet high academic standards. The organization was founded in 1921. Today, over 1 million students participate in NHS, which has chapters around the world. NHS emphasizes leadership and service.

What is the NHS for dummies? ›

The NHS stands for the National Health Service, which provides health care for all UK citizens based on their need for medical care rather than their ability to pay for it. The NHS was established in 1948 as one of the major social reforms following the Second World War and is funded by taxes.

Why does NHS matter? ›

The purpose of the National Honor Society is to elevate students' and schools' academics, leadership, and community engagement. NHS benefits students, communities, and colleges. Colleges have a way of seeing the academic and service commitment of an applicant through his or her membership.

How stable is NHS? ›

Although prepared NHS or Sulfo-NHS esters are sufficiently stable to process in a two-step reaction scheme, both groups will hydrolyze within hours or minutes, depending on water-content and pH of the reaction solution. (NHS esters have a half-life of 4-5 hours at pH 7, 1 hour at pH 8 and only 10 minutes at pH 8.6.)

What is NHS solution? ›

NHS Solutions focuses exclusively on interim executive healthcare leadership roles, both clinical and non-clinical. Our aim is to develop partnerships with our interim candidates and our hospital clients with the ultimate goal of providing excellent patient care across the United States.

What is the half-life of NHS? ›

The half-life of hydrolysis for NHS-ester compounds is 4 to 5 h at pH 7.0 and 0°C. This half-life decreases to 10 mins at pH 8.6 and 4°C. The extent of NHS-ester hydrolysis in aqueous solutions free of primary amines can be measured at 260 to 280 nm, because the NHS byproduct absorbs in that range.

How do you quench NHS ester? ›

NHS ester crosslinking reactions are most commonly performed in phosphate, carbonate/bicarbonate, HEPES and borate buffers. Other buffers may also be used, provided they do not contain primary amines such as Tris or glycine. Using a large excess of Tris or glycine at neutral-to-basic pH can quench the reaction.

What is EDC and NHS? ›

The EDC/NHS activation results for four small molecules of dicarboxylic acids in aqueous media, namely, glutaric acid and 2,2-dimethyl glutaric acid, which generate disuccinimidyl ester with high yield, and succinic acid and 2,2-dimethyl succinic acid, which remain intact, can also be explained by the Thorpe–Ingold ...

Why is my agarose gel melting? ›

Agarose gels are cast and run using TAE or TBE buffer. Since both buffers are clear liquids, it's easy to mistake them for water. If water is used, the gel will melt shortly after applying a charge to the gel box—say goodbye to those precious DNA samples!

How do you prevent smearing on agarose gel? ›

Gel preparation to help minimize smearing in gel electrophoresis. Keep the gel thickness around 3–4 mm when casting horizontal agarose gels. Gels thicker than 5 mm may result in band diffusion during electrophoresis.

Why is my DNA stuck in my agarose gel well? ›

The presence of DNA binding proteins in the sample, such as ligases, phosphatases or restriction enzymes may alter DNA migration in the gel and cause the DNA to remain in the gel wells. Lambda DNA or other DNA with long complementary overhangs may anneal resulting in an atypical band migration pattern.

Is agarose and Sepharose the same? ›

Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. Its brand name is a portmanteau derived from Separation-Pharmacia-Agarose.

What is the difference between Sepharose and agarose beads? ›

The answer is simple. It really doesn't matter as they are in fact the same. Sepharose is a tradename for a crosslinked, beaded-form of a polysaccharide polymer material extracted from seaweed. Its brand name is derived from Separation-Pharmacia-Agarose.

What is the difference between Sepharose and agarose? ›

1. Pure agarose is powdered form while sepharose is more beaded in structure. 2. Agarose is a more generic term referring to a type of polysaccharide polymer while sepharose is a trademarked term by GE Healthcare.

How does NHS pathways work? ›

NHS Pathways is a suite of clinical assessment content for triaging telephone calls from the public, based on the symptoms they report when they call. NHS Pathways enables a specially designed clinical assessment to be carried out by the trained person answering the call.

What is NHS reagent? ›

N-Hydroxysuccinimide (NHS) is an organic compound with the formula (CH2CO)2NOH. It is a white solid that is used as a reagent for preparing active esters in peptide synthesis. It can be synthesized by heating succinic anhydride with hydroxylamine or hydroxylamine hydrochloride.

What is shared purpose NHS? ›

Leaders create a shared purpose for diverse individuals doing different work, inspiring them to believe in shared values so that they deliver benefits for patients, their families and the community.

What is pierce recombinant protein A agarose? ›

Thermo Scientific Pierce Protein A Agarose is a standard-capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods. Pierce Protein A Agarose consists of purified native Protein A that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose.

What is BP agarose gel? ›

The term 'bp' in gel electrophoresis stands for base pair. Base pairs refer to a set of complementary nucleotides in DNA. A long strand of nucleotides linked together forms a DNA molecule. Thus, referring to the base pairs in a fragment tells the scientist how long, or how large a particular fragment is.

What is agarose gel and what is it used for? ›

Agarose gel electrophoresis has proven to be an efficient and effective way of separating nucleic acids. Agarose's high gel strength allows for the handling of low percentage gels for the separation of large DNA fragments.

What does agarose gel test for? ›

Agarose gel electrophoresis is a method used to separate DNA molecule(s) on the basis of their molecular weight. Stained agarose gels can be used to estimate the molecular weight and purity of the DNA fragment(s) in a given sample. The conformation of a DNA molecule can affect its migration through an agarose gel.

What is the difference between protein A and Protein G agarose beads? ›

Protein A and G are structurally very similar, but they have slightly different affinities for IgG subclasses across different species. These affinities overlap, but in general, protein A has greater affinity for rabbit, pig, dog, and cat IgG whereas protein G has greater affinity for mouse and human IgG.

What is protein A or G agarose beads? ›

The agarose beads have physical and chemical properties suitable for many affinity purification systems. Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli.

What is the difference between recombinant protein and DNA? ›

Recombinant DNA is a molecule of DNA made by combining genetic material from different species generating new genetic combinations. Recombinant protein refers to the protein that is translated by the host organism based on the information present in the recombinant DNA.

Why do you use 1% agarose gel? ›

The higher the percentage of agarose, the smaller the pore size, thus the smaller the molecules able to pass and the slower the migration. In the molecular biology lab, 0.7-1% agarose gel is typically used for day-to-day DNA separations, offering good, clear differentiation of fragments in the range of 0.2-10 kb.

What is the difference between agarose and agar? ›

Agarose is also used in the field of microbiology, but to a much lesser extent than agar. While Agar is extracted directly from the cell wall of different species of red algae (Gelidium, Gracilaria...), Agarose is obtained later from the agar through a complex and costly purification process.

Why is agarose better than agar? ›

Agarose is a result of purification of polysaccharide agar. In other words, agar is purified from agar by removing agaropectin in agar. Agarose is very beneficial to bacteria culture since it does not contain protein, food of the bacteria. An agarose is generally extracted from seaweed of agar.

Is agarose safe to use? ›

When taken by mouth: Agar is POSSIBLY SAFE for most adults when taken with at least one 8-ounce glass of water. If it is not taken with enough water, agar can swell and block the esophagus or bowel.

Is agarose gel positive or negative? ›

Introduction. Gel electrophoresis is the standard lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive electrode.

Why can we see DNA on agarose gels? ›

Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones. When a gel is stained with a DNA-binding dye, the DNA fragments can be seen as bands, each representing a group of same-sized DNA fragments.

What are the advantages and disadvantages of agarose gel electrophoresis? ›

The advantages are that the gel is easily poured, and does not denature the samples. The samples can also be recovered. The disadvantages are that gels can melt during electrophoresis, the buffer can become exhausted, and different forms of genetic material may run in unpredictable forms.


1. His-tagged Protein Purification
2. Gel Filtration Sephadex G 50
3. DEAE Sephacel Column: Protein Purification via Ion Exchange
4. How to Use the New ChemiDoc MP for Chemiluminescent Western Blot Imaging
(Bio-Rad Laboratories)
5. Strategies for successful crosslinking and bioconjugation applications
(Thermo Fisher Scientific)
6. KOD One™ PCR Master Mix
(Merck Life Science)


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