Affinity Conjugation Medium, NHS Activated Sepharose™ 4 Fast Flow (2023)


Purchase orders must be sent to VWR International S.r.l., Via San Giusto 85 - 20153 Milan, tel. No. 02-332031.1, free fax number. 800-152999, 02-332031307 or 02-40090010.

Telephone orders must be confirmed in writing, clearly stating: "Telephone Order Confirmation".

For orders sent by fax. Via email, traditional mail or telephone, the minimum order amount is EUR 200 (excluding VAT), below which an administration fee will be charged at the following rate: EUR 30.00 - (excluding VAT). These charges do not apply to online orders placed through the VWR International web shop (

When compiling an order, observe the VWRe PBI catalog nomenclature and specify exactly the code associated with the individual product, and consider indicating, where possible, the pack quantity equivalent to or a multiple of the standard pack.

Due to supply difficulties due to force majeure, we have the right to cancel all or part of the order or contract, either at our factory or at the factory of our suppliers.

We have the right to terminate the contract or suspend the supply if the economic situation of the buyer changes (bankruptcy, liquidation, insolvency, etc.)

packing and shipping costs

The merchandise is always delivered from our warehouse and transport is charged at cost price. The choice of packing or shipping method imposed on us by shipping laws and regulations is deemed to have been accepted by the customer in advance.

IMPORTANT NOTE: Ethanol, excise tax amounts (if due) are legally valid at the time the order is processed and must be added to the specified price. We specify that VAT is also paid on the GST value.

The contribution to shipping costs is a maximum of 25.00 euros (twenty-five) for each order received, regardless of the amount sent for the same order. For online orders sent via the VWR International webshop (, the contribution to shipping costs is 13 (thirteen) euros.

special transport

The shipping cost for DRY ICE is 38 EUR per shipment. The shipping cost of BLUE ICE dry ice is 26 EUR per lot. In the case of mixed shipments, one charge will be made for each type, which is the highest charge necessary.

The type of special transport will be quoted separately according to the weight, size and degree of danger of the product. Contact us 02-3320311 or by clarification.

standard packing

Buyers should be aware of issues related to safety, ease of storage, handling, etc. These problems can be avoided by purchasing standard containers of chemicals, especially hazardous chemicals, from:


Claims - Returns

Claims related to product quality issues or damage during shipping and delivery must be made in writing within 8 days of receipt of shipment, citing shipping document details. In no case will returns of products not expressly authorized by VWR International S.r.l. be accepted. Returned products must be accompanied by a delivery note containing a reference to the original shipping document and the reason for the return. With the prior authorization of VWR International PBI S.r.l, it is possible to return incorrectly ordered material, shipping costs are borne by the sender, and 80% of the value of the returned item is refundable, provided that the item is in perfect condition and in its original condition. original packaging. We remind you to always accept deliveries from couriers with reservations if you are unable to inspect the contents immediately after delivery.


The equipment is guaranteed for 12 months unless otherwise stated in the quote. During this period, those parts that may have manufacturing or operating defects will be replaced or repaired free of charge at the factory. Used or expired parts (such as various consumables, solutions, etc.) are not covered by warranty: if they are not fully functional, they must be returned for replacement within 8 days of arrival (see Claims). pre-approved, all returns including replacement or warranty repair will not be accepted.

The warranty starts from the date of shipment of the goods or the date of installation as agreed by both parties. If the installation is not carried out for reasons not attributable to VWR International, the start date of the guarantee period will automatically be set at 3 months from the date of shipment of the product.


It is at our headquarters in Milan unless agreed before the order. The cost of any tests outside of our office must be negotiated.

VWR International sales and service personnel are not authorized to accept any indemnification or release of liability on behalf of the company. During testing, VWR personnel will be limited to instructing the operator on the proper use of the instrument.

Performance of our technicians

They will be charged in accordance with our company's current rates for such services. No definitive invoice will be issued unless expressly accepted in writing by the Contractor.


Promotional offers cannot be used in conjunction with other discounts and/or offers of any kind.


The net price quoted includes the cost of standard packaging.

Prices quoted do not include VAT or GST.

The prices offered must be considered valid until the expiration of the offer document, beginning the period of validity in the same from the date of its publication.

VWR International S.r.l may change the prices in the price list without prior notice

price availability

The prices of some imported items are based on the exchange rate against the Euro at the time we compile our price lists, so prices may be adjusted if exchange rates change.

ORDINI Laboratory Products Catalog

Certain products included in the OMNIALAB catalog have a numeric code that can be changed in the operating system as of January 1, 2012. Please contact us according to the specific code you need.

payment terms

Unless otherwise stated in the order acceptance, the contractor agrees to remit all payments in full by bank transfer to the address indicated on the invoice from the date of the invoice or proof (if applicable).

If the payment is not remitted in its entirety within the agreed period, the buyer must pay an interest equal to that reported in Legislative Decree 231/2002.

For new clients that are not public institutions, an advance payment is required; any exceptions to these terms must be agreed directly with VWR International S.r.l.


If an agreement is not reached before ordering or quoting, the customer must indicate the desired shipping method. If it is not specified, VWR International will choose the most suitable shipping method, informing you of the amount.

Free port shipments are delivered by road in packaging suitable for the product.

Any other charges related to specific deliveries or lifting the equipment off the ground will be listed separately after inspection by our professional couriers (see Special Shipping paragraph).deliver goods

Delivery times are indicative and not binding.

technical characteristics

Device features may change without notice.


The products we supply are intended to be used in accordance with the manufacturer's documentation and specifications. Any use thereof is the sole responsibility of the user. However, for some specific products (APIs), VWR as a distributor cannot meet the requirements set out in the European GMP guideline for medicines for human and veterinary use and therefore cannot sell these products through the usual logistics channels. .

VWR Internationals.r.l., the national distributor, acts solely as a "broker" of the active substance, without storage, handling, repackaging, relabeling, etc., but guaranteeing full traceability of the transactions carried out.

The products we supply are not intended for use as active ingredients in human or veterinary medicines. For uses as food additives and pesticides in the pharmaceutical, cosmetic and agricultural sectors and for household use, customers should contact their local VWR organization.

Customers intending to use these products for pharmaceutical, cosmetic, food or any other purpose must establish all necessary internal controls and ensure that they comply with local and European regulations (eg European Pharmacopoeia). and meet your requirements.

VWR INTERNATIONAL is not responsible in any way for the use of products that are not suitable for their intended use.

It is the customer's responsibility to comply with applicable local health, environmental and safety regulations and to prepare the necessary actions related to the storage, handling, sale and use of the product.

privacy protection

In accordance with article 13 of the Legislative Decree. No. 196 of June 30, 2003, containing provisions on the subject "Protection of persons and other subjects with regard to the processing of personal data", VWR International S.r.l. The data obtained is with parties related to your company or institution and is only used for the promotion, celebration and execution of contracts. Only interested and expressly authorized persons will have access to your data.

The right to cancellation, anonymization, blocking, updating, rectification or integration of the processing data is valid in accordance with Section 7 of the aforementioned law.

Executive Forum

The courts of Milan will have exclusive jurisdiction for any dispute arising from the supply of the equipment indicated in the quote, the execution of the sales contract and the warranty service.

The general conditions of sale for online orders are automatically considered accepted at the time of order.


Affinity Conjugation Medium, NHS Activated Sepharose™ 4 Fast Flow? ›

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide

N-Hydroxysuccinimide (NHS) is an organic compound with the formula (CH2CO)2NOH. It is a white solid that is used as a reagent for preparing active esters in peptide synthesis. It can be synthesized by heating succinic anhydride with hydroxylamine or hydroxylamine hydrochloride. › wiki › N-Hydroxysuccinimide
) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

What is Sepharose resin? ›

Sepharose™ is a spherical, agarose-based size exclusion chromatography resin. Sepharose is available with 2 different agarose contents, 4% and 6%, designated Sepharose 4B and Sepharose 6B respectively.

What are Sepharose beads? ›

Sepharose Big Beads are ion exchangers designed for large-scale industrial applications. The media are based on 100–300 μM, cross-linked 6% agarose particles, substituted with quaternary ammonium (Q) or sulfopropyl (SP) groups.

What is Sepharose used for? ›

Abstract. Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.

What is the difference between Sepharose and agarose beads? ›

The answer is simple. It really doesn't matter as they are in fact the same. Sepharose is a tradename for a crosslinked, beaded-form of a polysaccharide polymer material extracted from seaweed. Its brand name is derived from Separation-Pharmacia-Agarose.

What is the difference between protein G and A beads? ›

Protein A and G are structurally very similar, but they have slightly different affinities for IgG subclasses across different species. These affinities overlap, but in general, protein A has greater affinity for rabbit, pig, dog, and cat IgG whereas protein G has greater affinity for mouse and human IgG.

How do Sepharose beads work? ›

Sepharose beads are porous, which gives them a high surface area for interaction with proteins and allows them to hold a lot of liquid. This is perfect for the application that they were originally designed for: purifying milligrams of protein in columns.

What are Sepharose beads charged with? ›

Ni Sepharose High Performance (HP) affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.

What does Sepharose bind? ›

Dextrin Sepharose™ High Performance is a chromatography medium for purifying proteins tagged with maltose binding protein (MBP). Thanks to the high specificity of the binding, very high purity is achieved in just one step.

What is protein A Sepharose 4 fast flow resin? ›

nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies. For the purification of monoclonal and polyclonal antibodies at both laboratory and process scale. Used in routine commercial polyclonal and monoclonal antibody purification and production.

What is chelating Sepharose fast flow packing? ›

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic, which comes immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms. The highly crosslinked agarose resin enables high flow rates and high productivity.

How do you elute protein from beads? ›

One of three methods can be used to elute the protein from the beads. SDS buffer is the harshest, which will also elute non-covalently bound antibodies and antibody fragments along with the protein of interest. On the other hand, glycine buffer gently elutes the protein with reduced amount of eluted antibody.

What does protein A Sepharose do? ›

Protein A-Sepharose® is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis.

What size are sepharose beads? ›

45-165 μm bead diameter.

Is protein A or G better for rabbits? ›

Protein A is generally preferred for rabbit, pig, dog and cat IgG. Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (IgG1, IgG2, etc.). Protein A/G is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G.

Are protein A or G beads better for IP? ›

Generally speaking, Protein A beads are better suited for rabbit antibodies, while protein G beads have higher affinity to mouse antibodies. Agarose and magnetic beads are two of the most common supports used in IP.

How many gs of protein is good? ›

According to the Dietary Reference Intake report for macronutrients, a sedentary adult should consume 0.8 grams of protein per kilogram of body weight, or 0.36 grams per pound. That means that the average sedentary man should eat about 56 grams of protein per day, and the average woman should eat about 46 grams.

What is the disadvantage of immunoprecipitation? ›

Limitations of IP

Co-IP might not be able to capture low affinity and transient protein interactions. The protein-protein interaction detected by co-IP may be indirect, because a third protein might be sandwiched in between the bait and prey proteins.

What beads bind antibodies? ›

Magnetic beads are used for biomagnetic separation procedures to enrich populations of a target cell, protein, or nucleic acid. Since the affinity between antibody and antigen is strong and specific, antibodies are often conjugated to the surface of magnetic beads in order to bind target cell or protein for enrichment.

Why are beads used in flow cytometry? ›

Compensation beads capture species-specific antibodies conjugated to fluorophores and other types of reagents. The purpose of these beads is to set voltages and gating parameters for obtaining accurate fluorescence signal.

Are Sepharose beads magnetic? ›

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni. His Mag Sepharose® Ni is a magnetic-bead-based IMAC medium charged with nickel ions.

How do you elute DNA from magnetic beads? ›

How does magnetic bead DNA extraction work? After binding a biomolecule, like DNA, an external magnetic field makes the beads stick to the outer edge of the containing tube. As the beads are immobilized, the bead-bound DNA is retained during washing. An elution buffer is added, and the magnetic field is removed.

How do DNA purification beads work? ›

Overview of magnetic bead-based DNA extraction using Sera-Mag beads. After binding DNA, an external magnetic field attracts the beads to the outer edge of the containing tube, immobilizing them. While the beads are immobilized, the bead-bound DNA is retained during the washing steps.

What is the difference between Sephadex and Sepharose? ›

Generally Sepharose is the result of evolution of Sephadex. There is different type of Sepharose, best of all will be SP Sepharose High Performance, which has Particle Size 24 µm-44 µm while SP Sephadex C-50 has 40 µm-120 µm. It's mean that separation by of Sepharose High Performance will be better.

What is lysine Sepharose affinity chromatography used to purify? ›

It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine. Lysine Sepharose 4B is a group specific adsorbent and has been used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA.

What is the resin in affinity chromatography? ›

Profinity Epoxide Resin is an activated affinity chromatography support for the immobilization of biomolecules that contain nucleophiles such as amino, thiol, or hydroxyl groups.

What is SP in chromatography? ›

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. Well established strong cation exchanger. Based on Sepharose Fast Flow ion exchange resin. High chemical stability enables proven CIP and sanitization protocols.

How do I activate Sepharose? ›

Activate the Sepharose beads in 20–50 ml cold 1 mM HCl for 15 min at 4°C. d. Wash the beads with 1 mM HCl. In general, 1 g of Sepharose beads requires 200 ml of HCl to wash.

What is IgG Sepharose 6 fast flow affinity resin? ›

lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins. Designed for rapid, single-step purification of protein A and protein A fusion conjugates. Binds at least 2 mg protein A/mL resin.

What does affinity chromatography remove? ›

The process is often used to purify biomolecules such as enzymes, antibodies, and recombinant proteins. It can also help to remove harmful substances such as pathogens through the same principles.

How can affinity chromatography improve purity? ›

Unlike other purification methods such as gel filtration and size-exclusion chromatography, affinity chromatography manipulates specific molecular properties and binding interactions between molecules to purify the protein of interest.

Which chromatography is best for protein purification? ›

Column chromatography is one of the most common methods of protein purification.

Why is affinity chromatography useful? ›

Why Use Affinity Chromatography? Affinity chromatography offers high selectivity, resolution, and capacity in most protein purification schemes. It has the advantage of utilizing a protein's biological structure or function for purification.

What is affinity chromatography What is it used for? ›

Affinity chromatography has been used for both sample preparation and as an analytical tool for the isolation or measurement of specific targets in biological, clinical and environmental samples [1–5]. In addition, this method has been utilized as a tool to study and characterize biological interactions [1,11–15].

Why is affinity chromatography expensive? ›

Affinity chromatography is the most expensive chromatographic method, since often a highly purified protein (the antibody) must also be manufactured before the target protein.

What elutes first in chromatography? ›

Since the adsorbents are polar, the more polar compounds are adsorbed more strongly. Thus, non-polar compounds are eluted first.

Do more polar solvents elute faster? ›

The higher the percentage of polar solvent, the faster compounds will elute.

How do you know if chromatography is successful? ›

In a successful chromatography experiment, the target molecules and impurities have different affinities for the stationary phase. Thus, one spends a longer duration on the column than the other, allowing separation to occur.


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